Plasticizers Increase Adhesion of the Deteriogenic Fungus Aureobasidium pullulans to Polyvinyl Chloride

[Survey of plasticizers in polyvinyl chloride toys].

Plasticizers in 101 samples of polyvinyl chloride (PVC) toys on the Japanese market were surveyed. No phthalates were detected in designated toys, though bis(2-ethylhexyl)phthalate, diisononyl phthalate, diisobutyl phthalate, dibutyl phthalate, diisodecyl phthalate and benzyl butyl phthalate were detected in more than half of other toys. 2,2,4-Tributyl-1,3-pentanediol diisobutylate, o-acetyl tr...

Antibacterial Activity of Aureobasidium Pullulans

Aureobasidium pullulans is ubiquitous, polymorphic and black fungus producing a variety of important metabolites including enzymes, polysaccharides and single cell protein. It is considered as a probable source for antagonistic activity against a number of phytopathogenic fungi and possible biocontrol agent of post harvest diseases. The present work aims at the antibacterial compounds produced ...

Genetic transformation of Aureobasidium pullulans.

Aureobasidium pullulans strain Y117 was transformed to hygromycin resistance using plasmid pDH33, which contains the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the Aspergillus niger glucoamylase gene (glaA). Southern hybridizations of transformants revealed multiple, integrated copies of the vector. The glaA promoter was not induced by starch in A. pullul...

CONTROL OF MOLECULAR WEIGHT DISTRIBUTION OF THE BIOPOLYMER PULLULAN PRODUCED BY THE FUNGUS Aureobasidium pullulans

Expression of green fluorescent protein in Aureobasidium pullulans and quantification of the fungus on leaf surfaces.

A red-shifted, mutated form of the jelly-fish green fluorescent protein (GFP) under control of a TEF promoter was expressed at high levels in the filamentous fungus Aureobasidium pullulans. In the three transformants studied, all morphotypes of the fungus, including pigmented chlamydospores, expressed GFP and fluoresced brightly. Confocal microscopy showed that the intra-cellular distribution o...